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Fig. 1. Characteristics of adult human mesodermal stromal cells (hMSC) and human marrow-derived neural stem cell-like cells (hmNSC) during expansion. (A) Morphology, fibronectin and nestin expression of hMSC (left panels) and hmNSC (right panels). All hMSCs express high levels of fibronectin, but only 4±3% of cells express very low levels of the NSC marker nestin, whereas some hmNSCs express low levels of fibronectin, but nearly all cells express high levels of nestin. Nuclei are counterstained with DAPI (blue). Bar, 100 µm. (B) Representative sequence of phase-contrast photomicrographs of hmNSCs 1, 5, 12 and 21 days after conversion from hMSCs. A growth curve of hmNSCs revealed by enumerating the cells at each time point under a hemocytometer is also shown (n=5; calculated doubling time 2.6 days). Bar, 50 µm.





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