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Fig. 5. Analysis of domains in SF3a60 required for nuclear and subnuclear localization in live cells. (A) Schematic representation of recombinant SF3a60 proteins fused to GFP. Amino acids present in, or deleted from the recombinant proteins are given in parentheses after the names of the proteins. Conserved regions are indicated above the diagram and numbered according to the amino acids in full-length SF3a60. (B) GFP-3a60 proteins with N- or C-terminal or internal deletions (as indicated) were transiently expressed in HeLa cells. Fluorescence was monitored in live cells. Bar, 10 µm.





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