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Fig. 6. Comparison of the distribution of transiently expressed GFP-3a60 proteins with SC35 and p80-coilin in fixed cells. (A-H) HeLa cells were transiently transfected with plasmids encoding GFP (A), GFP-3a60-FL (B), GFP-3a60-N1 (C), GFP-3a60-C4 (D), GFP-3a60-C3 (E), GFP-3a60-{Delta}Zn (F and G) and GFP-3a60-{Delta}SAP (H). Cells were fixed in cold methanol and immunostained with anti-p80-coilin (D and G) or anti-SC35 (E, F and H). The images in (A-C) and the left panels of (D-H) show fluorescence of GFP. The middle panels of (D-H) show immunolocalization of p80-coilin or SC35, and the right panels represent computer-generated overlays of the two images. Bar, 10 µm.





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