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Fig. 4. Hyperacetylated histone H4. Light and fluorescent micrographs of mouse immature oocytes, fertilized eggs and early embryonic stages stained for the hyperacetylated H4 modification (F-J, red) and nucleic acid (K-O, green). (A,F,K) In immature oocytes, hyperacetylated H4 staining is limited to condensing chromatin surrounding the nucleolus. Cumulus cells are also strongly stained by this antibody (data not shown). (B,G,L) In fertilized eggs, staining for this modification is seen in decondensing sperm nuclei (G, arrows) and is only weakly associated with the metaphase plate of maternal chromatin (G, asterisk). (D,I,N) At the four-cell stage almost no staining is observed in metaphase stage blastomeres (arrows) whereas strong staining is seen in interphase stage blastomeres. (E,J,O) However, by the blastocyst stage of development, hyperacetylated H4 staining again becomes weakly associated with metaphase chromatin (arrows). Fluorescent images represent single confocal sections, therefore only nuclei found within the optical section can be visualized.





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