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Fig. 5. Methylated arginine 17 on histone H3. Light and fluorescent micrographs of mouse immature oocytes, fertilized eggs and early embryonic stages stained for the Me(Arg17)H3 modification (F-J, red) and nucleic acid (K-O, green). (A,F,K) Immature oocyte nuclei displayed high levels of this modification in a punctate manner throughout the entire nuclear region excluding the nucleolus. (B,G,L) At fertilization, diffuse punctate cytoplasmic Me(Arg17)H3 staining is seen with no apparent staining observed on the metaphase plate (asterisks). (C,H,M) At the pronuclear stage, however, the modification is associated with chromatin in both pronuclei at similar levels. In all of the subsequent developmental stages tested, punctate cytoplasmic staining with little or no chromatin staining is observed in metaphase stage blastomeres (J, O, arrows). There is also little or no staining in anaphase stage blastomeres (I, N, arrows). This modification also stains cumulus cell nuclei and only stains polar bodies faintly (data not shown). Fluorescent images represent single confocal sections, therefore only nuclei found within the optical section can be visualized.





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