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Fig. 6. Methylated arginine 3 on histone H4. Light and fluorescent micrographs of mouse immature oocytes, fertilized eggs and early embryonic stages stained for the Me(Arg3)H4 modification (B,E,I,L,O,R, red) and nucleic acid (C,F,J,M,P,S, green). (A-C) This modification is also found in a punctate manner throughout the nucleus of the immature oocyte. (D-F) At fertilization, this modification appears to be particularly affected by the egg cytoplasm, as it is undetectable in both the egg cytoplasm and the metaphase plate chromatin of fertilized oocytes (asterisks). (H-J) At the pronuclear stage, nuclear Me(Arg3)H4 staining becomes apparent in some zygotes (data not shown) whereas other zygotes do not appear to stain positive for this modification. (N-P) Staining becomes evident with increasing levels in interphase stage blastomeres at all subsequent developmental stages. (K-M) Weak staining is seen associated with chromatin in cleavage stage metaphase stage blastomere. (Q-S) However, by the blastocyst stage of development the Me(Arg3)H4 modification does appear to partially re-associate with the metaphase stage chromatin (arrows). Fluorescent images represent single confocal sections, therefore only nuclei found within the optical section can be visualized.





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