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Fig. 2. Accumulation of cytoplasmic SGs in a variety of experimental conditions. (A) Effect of genetic invalidation of the AIF gene. Male control ES cells or cells in which the AIF gene (on the X chromosome) has been invalidated (AIF^–/y) were subjected to HAsO₄ treatment (1 mM, 3 hours), followed by fixation, permeabilization and immunostaining for the detection of TIA-1 (red) and AIF (green, shown in the insets). (B) Redistribution of TIA-1-GFP into SGs in AIF-negative cells. HeLa cells treated with siRNA for the downmodulation of AIF (or controls) for 48 hours and then transfected with TIA-1-GFP were treated with HAsO₄ during the last 3 hours of the experiment and then subjected to fixation and staining for AIF (red). (C) Redistribution of PABP-GFP into SGs in AIF-negative cells. The protocol was similar to Fig. 2B, with the difference that cells were transfected with PABP-GFP instead of TIA-1-GFP. Representative cells are depicted in the microphotographs and the percentage (±s.d., n=5) of SG⁺ cells was plotted in the right panels.

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