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Fig. 6. Effect of AIF on redox metabolism. ES cells positive or negative for AIF (A,B,D,F) or HeLa subjected to AIF knock-down (C,E) were treated with HAsO4 (A,B) or a panel of different pro-oxidants (and as an internal control for GSH depletion with BSO) for 1-3 hours (A,B) or 3 hours (C-F). Then, the level of NAD(P)H was determined by cytofluorometry, based on its autofluorescence (A,C-F). Alternatively, the cells were stained with the GSH-reactive fluorochrome monochlorobimane (MCB, B,C-F). Results are expressed as the specific variation obtained in triplicate determinations (+s.d.) in one experiment representative of three. PQT, paraquat.





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