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Fig. 5. Focal adhesion and actin stress fiber formation in cells expressing MARCKS mutants. (A) Focal adhesion sites were labeled with an anti-FAK antibody 2 hours after cells were plated on fibronectin. Control cells were transfected with empty vector. (B) Cells were plated on fibronectin for 2 hours, fixed, and stained with Phalloidin Alexa488 to label stress fibers. (C) Quantification of actin polymerization by sedimentation assay in cells expressing MARCKS fusion proteins. Cell extracts were analyzed by western blotting for total actin and the amount of actin in the G-actin pool or the F-actin pool. Bars, 2 µm.





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