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Fig. 11. Noncleavable and precleaved lam5 promote phosphorylation of focal adhesion kinase. As indicated, HFKs or {alpha}3–/– MKs were suspended with trypsin then either left in suspension or re-adhered to the indicated surfaces (collagen, HFK, {alpha}3+/+, etc.) for 30 minutes. Triton X-100 extracts were prepared containing 100 µM Na3VO4, 10 mM NaF, PMSF and NEM from the adherent cells and suspended cells. Extracts were assayed for phosphorylated focal adhesion kinase (P-FAK) by immunoblotting with antibody FAK P-Y397.





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