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Fig. 6. Mutations in recombinant human laminin 3 chain. Wild-type and mutated human laminin 3 chains. Numbering of amino acid residues is from Ryan et al. (Ryan et al., 1994). Noncleavable 3 contains a 47 amino acid internal deletion from residue S1308 to residue C1354, in the spacer region between the G3 and G4 domains, producing a sequence in which C1307 is followed directly by S1355. This modification prevents cleavage of the G4/5 domains in culture. Precleaved 3 is a truncated 3 chain in which the G4 and G5 domains are not included, mimicking the cleavage that removes the G4/5 domains. The sequence stops at residue H1364, and five additional histidine residues have been added as an affinity tag at the C-terminus.

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