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Fig. 7. Immunoblotting of recombinant chimeric human/mouse lam5 deposited by ${alpha}$ 3–/– MKs. ECM from confluent cultures of ${alpha}$ 3–/– MKs (lane 3), ${alpha}$ 3+/+ MKs (lane 4) and ${alpha}$ 3–/– MKs expressing noncleavable (lane 5) or precleaved (lane 6) human ${alpha}$ 3 chains. Control samples of HFK lam5 containing ${alpha}$ 3²⁰⁰ chain (lane 2) or ${alpha}$ 3¹⁶⁵ chain (lane 1). Samples were fractionated by SDS-PAGE (A and B 5%, C 10%) and immunoblotted. (A) Mab P5H10 specific for the human ${alpha}$ 3 chain; (B) goat polyclonal antibody anti-ß3 chain; (C) Rabbit Mab R5808-F1 specific for G4/5 domain of mouse laminin ${alpha}$ 3²⁰⁰.

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