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Fig. 8. Expression of recombinant lam5 rescues adhesion and survival. {alpha}3+/+, {alpha}3–/–, {alpha}3–/– expressing noncleavable or precleaved human {alpha}3 chain were plated at 104 cells/well in 24-well Petri plates. Growth and deposition of the human {alpha}3 chain were monitored over a 7 day period. (A) Cell numbers were compared by counting 12 separate fields for a 10x objective. (B) ELISA for human {alpha}3 chain deposition onto the Petri dishes. (C) Phase-contrast images of cells after 7 days on Petri dishes. Bar, 25 µm. (D) Quantitation of cells in S-phase of the cell cycle after 48 hours on a Petri dish as determined by FACs analysis.





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