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Fig. 9. HFK adhesion to recombinant lam5 and effects of anti-integrin inhibitory antibodies. Trypsin suspended HFKs were plated into 24-well plates that are coated with type I collagen (COL.) or ECM from HFKs, {alpha}3+/+ MKs, {alpha}3–/– MKs, or {alpha}3–/– MKs expressing noncleavable and precleaved lam5. HFKs were allowed to adhere to the surfaces for 30 minutes at 37°C with or without the indicated Mabs: anti-LAM5 C2-9 against human laminin {alpha}3 chain; integrin ß1, P4C10; integrin {alpha}3, P1B5; integrin {alpha}2, P1H5; integrin {alpha}5, P1D6. Adhesion is reported as the proportion of fluorescently labeled cells remaining adhered after washing. Each condition was assayed in triplicate. This is a single adhesion assay, the results of which are representative of results obtained on at least three occasions.





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