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Fig. 10. Ykt6 exhibits a very broad vesicular cytoplasmic localization in cultured hippocampal neurons. Hippocampal neurons and glia from 1-4-day-old rats were cultured for 15 days in vitro before fixation and staining with anti-rat-Ykt6 (red) and anti-Sec6 (green) antibodies, and analysed by confocal microscopy. (B-E) Individual synaptic terminals with the plasma membrane clearly demarcated by Sec6 staining. Scale bar, 25 µm (A), 2 µm (E).





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