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Fig. 5. Rat Ykt6 localization is regulated by similar longin-domain surface features. Mutations to the longin domain were introduced to full-length rat Ykt6 and truncated rat Ykt6 composed of only a longin domain (residues 1-137). All the mutants were expressed in PC12 cells and the subcellular localization of each mutant was examined by fluorescent microscopy using anti-Myc antibody (9E10) and FITC-labeled secondary antibody. (Top) Mutants in the full-length context. (Bottom) Mutants in the isolated Ykt6 longin constructs. Scale bar, 10 µm.





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