spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. KCNE proteins are not determinants for apical and/or basolateral targeting of the KCNQ1 channel. (A) MDCK cells transiently transfected with KCNQ1+KCNE1 (Q1+E1), KCNQ1+KCNE2 (Q1+E2), KCNQ1+KCNE3 (Q1+E3), KCNQ1+KCNE4 (Q1+E4) or KCNQ1+KCNE5 (Q1+E5). The cells were fixed and labeled for KCNQ1. The top panel shows horizontal confocal scans through the cell layer at the basal level, whereas the bottom panel shows vertical confocal scans of the same cells. Ap, apical membrane, Ba, basal membrane. Bar, 8 µm. (B) Verification of protein expression of KCNE1-5 and KCNQ1 was performed by electrophysiological measurements. Whole-cell patch-clamp analyses were done as described for KCNQ1 alone, except for KCNQ1/KCNE5, where the depolarising pulses were extended to +140 mV.





Right arrow Return to article