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Fig. 6. Subcellular localisation of Y111A-KCNQ1 and Y51A-KCNQ1. MDCK cells transfected with Y111A-KCNQ1 (a-c) or Y51A-KCNQ1 (d-f) were biotinylated at the basolateral membrane (b) or the apical membrane (f2). The cells were fixed and labelled with KCNQ1 antibody (a,d,e,f1) and Alexa Fluor 488-streptavidin (b,f2). Horizontal confocal scans through the cell layer at the basal level are shown in a1-c1,d, whereas e shows a horizontal scan at the apical level of the same cells depicted in d. (a2-c2,f1-3) Vertical scans. (c,f3) Overlays of images a,b, and f1,f2, respectively. Y111A-KCNQ1 is located intracellularly, whereas Y51A-KCNQ1 displays a non-polarised distribution pattern in the plasma membrane. Ap, apical membrane, Ba, basal membrane. Bar, 8 µm.





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