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Fig. 8. Y51 is a dominant basolateral targeting signal. Expression of p75* and the chimeric constructs p75*-Q1(Y51) and p75*-Q1(Y51A) in MDCK cells. They were studied by confocal microscopy using a monoclonal antibody directed against p75. p75* is localised in the apical membrane of MDCK cells, whereas the chimeric construct p75*-Q1(Y51) is mainly located in the basolateral membrane. Mutation of Y51 into an alanine eliminates the ability of the KCNQ1 sequence to redirect p75* as p75*-Q1(Y51A) is located in the apical membrane. Both horizontal confocal scans through the cell layer and vertical confocal scans of the same cells are shown. For p75* and p75*-Q1(Y51A) horizontal scans through the cell layer at the apical level is illustrated, whereas a horizontal scan at the basal level is included for p75*-Q1(Y51). Ap, apical membrane, Ba, basal membrane. Bar, 8 µm.





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