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Fig. 6. Expression of Dab1RFP in NBT-II cells alters CrkII distribution but does not alter phosphorylation of Fak at Y397. (A) Endogenous CrkII (green) was observed to co-localize (yellow areas) with Dab1RFP (red) at the cell membrane of transfected NBT-II cells. (B) In cells expressing Dab1RFP-5F (red) the CrkII signal was not observed to overlap. (C) In cells expressing Dab1RFP, Fak phosphorylated at Y397 (green) was observed in the plane closest to the coverglass. (D) Dab1RFP-5F-transfected cells also expressed phosphorylated Fak.





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