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Fig. 2. Kinectin intracellular localisation in PC12 cells as revealed by anti-kinectin antibodies. (A) Staining using anti-CT1, reactive to all kinectin isoforms, reveals reticular cytoplasmic labelling. (B) Staining with anti-insert V2 antibody, reactive to insert V2-containing isoforms, results in cytoplasmic reticular labelling as in A, plus distinct labelling of the tips of neurite-like processes (arrow heads). (C) No labelling occurs using pre-immune serum. (D1-D3) Double immunofluorescence localisation of kinectin isoforms and the ER. (D1) Labelling using anti-insert V3 antibody, reactive to insert V3-bearing isoforms, again results in cytoplasmic reticular staining plus staining of neuritic processes (arrowheads); (D2) anti-PDI antibody to visualise the ER; (D3) overlay of the two images. Bars, 5 µm.





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