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Fig. 4. Roles of 160 kDa and 120 kDa kinectin in ER and mitochondria dynamics. (A) Localization of 160 kDa and 120 kDa kinectin. (a) HeLa cells, transfected with the GFP-TM construct, showed a distinct ER-like network staining. (b) Transient expression of N-terminally truncated non-TM construct, representing 120 kDa kinectin, showed in contrast, strong organelle-like aggregates and occasionally faint ER-like network labelling (arrow). Bar, 10 µm. (B) Effects of overexpression of TM domain on ER and mitochondria distribution in HeLa cells. ER network was labelled by anti-calreticulin mAb (red). Cells transfected with the TM construct were identified with GFP expression (green). (a) An enrichment of TM-containing protein was observed over the area where ER staining localized. (a') ER network integrity was affected after prolonged expression of the TM construct. Mitochondria were labelled with anti-Mn SOD (red). (b,b') Cells transfected with the TM construct (green) showed normal mitochondrial distribution. Bar, 20 µm.





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