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Fig. 5. Effects of overexpression of kinectin-kinesin binding domains on ER distribution in HeLa cells. (A) Typical images. Anti-calreticulin mAb was used to label the ER network (red). Transfected cells were identified by GFP expression (green). The network was normally distributed in a and a', representing GFP-only transfected cells (control). A collapsed ER network distribution was observed in KNT+ transfected cells (b,b'). Bar, 20 µm. (B) Quantification. The values shown represent the mean±s.e.m. of three experiments. A total of 90 cells were analysed per construct.
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