JCS01331 Supplemental Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Phase-contrast time-lapse sequences of control and ILK siRNA-transfected cells. Movies 1 and 2 were recorded on control (left) and ILK-depleted cells (right) 36 hours after the second transfection. Images were acquired at 3-minute intervals 2 hours after plating cells on fibronectin-coated dishes in complete medium (5% CO₂, 37°C).

• Supplemental Figure 2 -

  Fig. S2. Effect of ILK silencing on avb3 integrin localization and cell adhesion. The distribution of avb3 was visualized by immunostaining of control or ILK siRNA-transfected cells plated on vitronectin-coated coverslips. Scale bar, 20 mm.

• Supplemental Figure 3 -

  Fig. S3. Distribution of a5b1 integrin following adhesion of control or ILK siRNA-transfected cells for the indicated times to fibronectin-coated coverslips. a5b1 integrin was visualized by immunostaining with the anti-a5 polyclonal antibody. Scale bar, 20 mm.

• Movie 1
• Movie 2 -

  Movies 1 and 2. Phase-contrast time-lapse sequences of control and ILK siRNA-transfected cells. Movies 1 and 2 were recorded on control (left) and ILK-depleted cells (right) 36 hours after the second transfection. Images were acquired at 3-minute intervals 2 hours after plating cells on fibronectin-coated dishes in complete medium (5% CO₂, 37°C).