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Fig. 1. Characterization of LIS-1 antibodies. (A) Western blots of protein extracts from wild-type, lis-1(RNAi) and lis-1(t1698) embryos, probed with LIS-1 antibodies and reprobed with ${alpha}$ -tubulin (TUB) antibodies. We detected a $~$ 15 kDa band in some extracts from mixed developmental stages of homozygote lis-1(t1698) mutant animals (not shown). (B) Wild-type, lis-1(RNAi) and lis-1(t1550) embryos stained with LIS-1 and ${alpha}$ -tubulin antibodies. Wild-type embryos fixed and stained on the same slides as lis-1(RNAi) or lis-1(t1550) embryos are shown. Bar, 10 µm. (C) Quantification of average LIS-1 reactivity, expressed as a fraction of wild-type values for wild-type (100%, n=18), lis-1(RNAi) (5.2%; n=9) and lis-1(t1550) or lis-1(t1698) [referred to collectively as lis-1(mutant)] (2.9%; n=11) embryos.

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