QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. GFP–LIS-1 localizes to kinetochores independently of dynein. (A-C,E-G) Spinning-disc confocal time-lapse fluorescence microscopy of one-cell-stage embryos derived from homozygous lis-1(1550) adults expressing GFP–LIS-1 with no further treatment (A), after icp-1(RNAi) (B), hcp-4(RNAi) (C), dhc-1(RNAi) (E), dnc-1(RNAi) (F) or dnc-2(RNAi) (G). A single image during late prometaphase or equivalent stage is shown here; see also corresponding Movies 9, 13, 14, 17, 18 and 19 (in supplementary material, http://jcs.biologists.org/cgi/content/full/117/19/4571/DC1). Insets show 2.5-fold digitally magnified views of the region encompassing chromosomes. Note that enrichment in the vicinity of chromosomes during prometaphase is absent in hcp-4(RNAi) embryos (C), but is present in all other cases. Six to twelve embryos were imaged for each genotype, with a similar outcome. (D) Images from confocal time-lapse fluorescence microscopy of dhc-1(RNAi) one-cell-stage embryo expressing GFP-TUB and GFP-HIS (see also corresponding Movie 16, in supplementary material). Time elapsed is shown in minutes and seconds. Arrows indicate position of chromosomes derived from the male pronucleus. Arrowheads point to the single centrosome visible in this focal plane.

Return to article