spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. NTF-NgR generation is inhibited by BFA and occurs at the cell surface. (A) SH-SY5Y cells expressing NgR were incubated for 6 hours in OptiMEM in the absence (–) or presence (+) of 5 µM BFA. NgR was detected in the cell lysate and medium using the anti-V5 antibody. (B) Cells were surface biotinylated at 4°C and then incubated at 37°C in OptiMEM for the indicated times. Biotinylated proteins in the lysate and medium were precipitated with streptavidin-agarose and NgR detected with the anti-V5 antibody.





Right arrow Return to article