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Fig. 4. Volume measurements of transgene arrays in ß-galactosidase-expressing (blue dots) and non-expressing (white dots) cells. Each dot represents one nucleus. Dots above each other represent nuclei from the same image stack, thus excluding differences in local specimen conditions or image recording between these nuclei. (A) GFP/lac-repressor signals in PALZ39E cells from an inducible culture. Signals were recorded either from uninduced, non-expressing or induced, expressing cells. (B) FISH signals from an inducible PALZ39E culture that was either not induced (left) or induced with HMBA (right). (C) Uninducible PALZ39E cells. (D) PALZ39M cells.





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