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Fig. 5. Increase in transgene array volume associated with transcriptional activation is also observed using FISH. (A) PALZ39E nuclei with FISH signals for the transgene (green) and the centromeres (red). DNA counterstain in blue. Inset shows corresponding X-Gal staining. (B) PALZ39M nuclei with FISH signals for the transgene (green) in PALZ39M cells. Inset shows X-Gal staining. (C-F) Nuclei from PALZ39E cells without induction and X-Gal-negative (C,D) or with induction and X-Gal-positive (E,F). Colors as in (A). Fluorescence images are projections of confocal sections. Scale bar is for all fluorescence images.





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