|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Different radial distribution of active (blue) and inactive (gray) transgene arrays within interphase nuclei. Each nucleus was divided into 25 shells of equal thickness. 25 points on each curve show the percentage of the signal (transgene or counterstain) within each shell. The x-axis indicates the position of the center of the shell relative to the nuclear radius (which is defined as 100 units). Detection of the transgene in inducible PALZ39E cells was with GFP/lac-repressor (A; n=40 for expressing cells, n=12 for non-expressing cells) or by FISH (B; n=22 for expressing cells, n=25 for non-expressing cells). Signals in PALZ39M cells (C; n=22 for expressing cells, n=40 for non-expressing cells) were detected by FISH. Distribution of nuclear DNA is shown in red (from nuclei with active transgenes) and pink (from nuclei with inactive transgenes) for comparison. Red and pink curves are very similar, in (C), they completely overlap, so that the latter is not visible. Error bars indicate the standard deviation of the mean.
|
