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Fig. 7. Organization of actin cytoskeleton depends on localization of either FAK or FAK/PYK2 chimeras in focal adhesions. (A) The rate of actin polymerization in vitro is similar using lysates of cells that do (FAK+/+, DA2) and cell that do (FAK+/+, DA2) and cells that do not (FAK-/-) have FAK in focal contacts. (B) Cells that lacked FAK-family kinase activity in focal adhesions and had disorganized FN matrix (FAK-/-, SE6, SX4) were also unable to organize stress fibers into a parallel pattern (red, actin; blue, nuclei).





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