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Fig. 4. Condensation of gonosomal chromatin starts at zygotene/pachytene transition in spread spermatocytes (blue, DAPI; red, anti-SCP3; green, anti-histone H4). At leptotene (not shown) and zygotene (A-D) histone H4 showed uniform nuclear distribution. At zygotene/pachytene transition (E-H) histone H4 is slightly more concentrated in the forming XY-body located at the nuclear periphery. At mid-pachytene (I-L) and diplotene (not shown) the XY-bodies have a higher histone H4 concentration than the autosomal chromatin. Scale bars: 10 µm.





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