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Fig. 4. nimU is an essential gene. (A) Wild type, nimU24 and nimU{Delta} spores were grown for 14 hours at permissive and restrictive temperatures for nimU24, then fixed and stained with DAPI. The wild-type strain grew normally at both temperatures, producing multinucleate hyphae. nimU24 spores produced multinucleate hyphae at the permissive temperature, but only grossly enlarged nuclei at the restrictive temperature. The nimU{Delta} spores produced grossly enlarged nuclei at both temperatures.





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