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Fig. 7. Mitotic arrest but premature spindle elongation in nimU24 bimE7 cells. (A) bimE7 and nimU24 bimE7 cells were germinated overnight at the permissive temperature and then shifted to the restrictive temperature and fixed at 0.5 hourly intervals. The chromosome mitotic index was determined after staining with DAPI. (B) The same samples were also stained for mitotic spindles (anti-{alpha} tubulin immunofluorescence) and 100 mitotic spindles were measured for each time point using IP Lab software. Values plotted are arbitrary units and represent the mean and standard error for the 100 measurements.





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