QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Relationship between extracellular calcium and tyrosine phosphorylation. (A) Spermatozoa were incubated in BWW -Ca²⁺ (lane 1), or BWW -Ca²⁺ supplemented with 50 (lane 2), 100 (lane 3), 300 (lane 4) or 600 (lane 5), µM CaCl₂. After 120 minutes incubation, the cells were centrifuged, washed and lysed in 2% SDS. 1 µg of each lysate was loaded onto a 10% polyacrylamide gel. Tyrosine phosphorylated proteins were detected by western blot analysis using the anti-phosphotyrosine antibody 4G10. The positions of the molecular mass markers (kDa) are shown on the left hand side. (B) The nitrocellulose membrane was stripped at 65°C with shaking. Following this, the membrane was blocked and re-probed using -tubulin antibody. The western blot is representative of three similar experiments.

Return to article