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Fig. 1. Usherin colocalizes with type IV collagen. (A) A mid-modiolar cross section of murine cochlea was subjected to dual immunofluorescence staining using anti-usherin and anti-type IV collagen-specific antibodies. (B) Immunogold colocalization of usherin and type IV collagen. Testis was embedded in Unicryl embedding resin and ultrathin sections were reacted with antibodies specific for either usherin (rabbit anti-usherin) or type IV collagen (goat anti-collagen IV). Sections were then reacted with both 10 nm gold-conjugated anti-rabbit secondary antibodies and 5 nm gold-conjugated anti-goat secondary antibodies. Arrows denote typically observed colocalization for these two antibody probes. Scale bar: A, 50 µm; B, 0.2 µm.





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