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Fig. 4. The LE domain of usherin interacts with type IV collagen. Extracts of matrix from the indicated mouse tissues were (A) reacted with the fusion peptide consisting of the LE domain, immunoprecipitated with anti-GST antibodies, and the immunoprecipitate western blotted using anti-type IV collagen antibodies, or (B) directly immunoprecipitated with anti-type IV collagen antibodies and the immunoprecipitate western blotted using anti-usherin antibodies. The molecular mass markers are given in kilodaltons.





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