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Fig. 5. The LE domain of usherin interacts with the 7S domain and the triple helical domain of type IV collagen. Direct ELISA determined interaction between the domain-specific usherin fusion peptides and the indicated domains of type IV collagen. Fusion peptides were reacted with purified collagen domains, which were immobilized on plastic microtiter dishes. Standard deviations are given for triplicate measurements. TIV protomer, the full-length trimeric protomer of type IV collagen; TH, the triple helical domain of type IV collagen; NC1, the non-collagenous domain of type IV collagen.





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