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Fig. 1. N-cadherin adhesion, typical cell spreading and cytoskeleton organization on the Ncad-Fc substrate. (A) Myogenic mouse C2 cells expressing endogenous N-cadherin were spread on laminin (/Laminin), stained for ß-catenin and analysed by confocal microscopy. Alternatively, actin-GFP-transfected cells were spread on laminin and analysed by confocal microscopy in combination with differential interference contrast (DIC) imaging. Note the radial distribution of ß-catenin and the more complex actin network (circles) at cell-cell contacts. (B) C2 cells dissociated in trypsin-free conditions were seeded in serum-free medium on silanized glass coverslips coated by immunoadsorption with purified Ncad-Fc (/Ncad-Fc). Cells spread for 2 hours exhibited typical fried egg morphology (DIC). Cytoskeleton organization was assessed by labelling actin filaments with phalloidin and microtubules with anti-tubulin antibodies. Bar, 10 µm.
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