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Fig. 2. Cell spreading on Ncad-Fc induces the formation of cadherin adhesions. (A) C2 cells spread for 2 hours on Ncad-Fc were immunolabelled for ß-catenin, 
-catenin and p120 or directly observed for the localization of transfected N-cadherin-GFP. The three catenins as well as N-cadherin presented the same radial distribution in the lamellipodium. The radial distribution of ß-catenin was specifically detected on a 500 nm thin confocal section taken at the ventral side of fried-egg-shaped cells (confocal). The strong vesicle-like immunostaining in the central area of the cells was not detected in this section. (B) Double staining for ß-catenin (red) and F-actin (green) was performed on cells spread on Ncad-Fc and analysed by confocal microscopy. (C) Co-localization of the red (ß-catenin) and green (F-actin) signals was analysed along the line indicated on the overlay image by line scan (Metamorph software). An optical section taken close to the cell-substratum interface revealed a zone of coincidence of ß-catenin-positive structures and F-actin labelling within the lamellipodium. Bars, 10 µm.
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