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Fig. 3. Laminin or anti-N-cadherin antibodies failed to induce cadherin adhesions. (A) C2 cells were seeded on laminin (/Laminin) or anti-N-cadherin antibodies (/Anti-Ncad) and immunostained for ß-catenin or F-actin. The usual elongated shape of C2 cells grown on laminin was also observed on the anti-N-cadherin substrate. In both conditions, ß-catenin immunostaining was uniformly distributed at the cell surface and no radial distribution of ß-catenin and actin fibres was detected. Bar, 10 µm. (B) Quantitative analysis of cell shape achieved after 2 hours of spreading on the Ncad-Fc, laminin, anti-N-cad and poly-ornithine (PO) substrates; the number of counted cells was superior to 250 for each condition and repeated in three independent experiments. (C) Quantification of mouse C2 cells or S180 chicken N-cad cells forming cadherin adhesions when seeded on Ncad-Fc, polyclonal anti-mouse N-cadherin serum, laminin or monoclonal GC4 anti-chicken N-cadherin antibody, respectively (at least 250 cells counted in two independent experiments).
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