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Fig. 8. Co-enrichment of p120 and Rac1 in membrane fractions upon JMD-cad expression. C2 cells were transiently transfected with GFP (lane 1), p120-GFP plus JMD-cad (lane 2), JMD-cad (lane 3) or p120-GFP (lane 4), then cultured for 16 hours, harvested and submitted to subcellular fractionation. (A) Membrane-associated and cytosolic proteins (10 µg and 20 µg, respectively) were separated on 7% SDS-PAGE and blotted with anti-p120, anti-ß-catenin antibodies, then with anti-tubulin antibodies as a loading control. The results are representative of two independent experiments. (B) The same extracts were separated on 13% SDS-PAGE and immunoblotted against Rac1. (C) The JMD-cad expression induced a threefold increase in Rac1 recovered in membrane fractions. The densitometry analysis was performed with the NIH Image 2.0 software on two independent experiments.

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