spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Inhibition of the prolactin signalling pathway by colchicine. Primary mammary epithelial cells were cultured on basement membrane and exposed to colchicine for the indicated times. Prolactin was added to the cultures 15 minutes before harvesting the cells as in Fig. 3. (A) Nuclear extracts were analysed by electrophoretic mobility shift assay for Stat5-DNA interactions. (B-E) Detergent lysates were immunoprecipitated for Stat5 (B,C), Jak2 (D) or PrlR (E) and immunoblotted with antibodies for phosphotyrosine (4G10) or the appropriate precipitating antibody.





Right arrow Return to article