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Fig. 1. RNAi of VCP and VCP adaptor proteins in Drosophila S2 cells. Drosophila S2 cells were subjected to RNAi of the indicated proteins. (A) The mRNA levels of indicated RNAi targets were determined by semiquantitative reverse-transcription PCR 4 days after the addition of indicated dsRNAs. (B) Equal amounts of whole-cell lysate protein were subjected to western blotting using antibodies against ubiquitin, dVCP and actin.





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