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Fig. 5. RNAi of VCP induces vacuolization of the cytoplasm. HeLa cells were subjected to RNAi of VCP for 4 days and/or treated with 10 µM MG132 for 6 hours. Immunofluorescence confocal microscopy was conduced for VCP (red) and for p62 (an ER marker protein), ß-COP (a cis-Golgi marker protein), TGN46 (a trans-Golgi marker protein) and Rpn12 (a subunit of the 26S proteasome) (green). Merged images are shown. Single channel images are available online (Figs S1, S2, S3 and S4, http://jcs.biologists.org/supplemental/). Scale bar, 20 µm.





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