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Fig. 8. RNAi of VCP induces apoptosis. (A) HeLa cells were subjected to RNAi of VCP for 2 days or 4 days. Equal amounts of protein from whole cell lysates were subjected to western blotting for the indicated proteins. (B) HeLa cells were subjected to RNAi for the indicated proteins for 4 days or treated with 5 µM MG132 for 6 hours. Caspase 3/7 activity was measured in each group of cells. Activity from control cells was assigned a value of 100% and activities from experimental groups are expressed as a percentage of the control. Results represent the mean (±s.e.m.) of three independent experiments. Each experimental group was compared to the control by Student's t test. The asterisk indicates P<0.05.





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