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Fig. 2. Immunocytochemistry (ICC) showing high efficacy of recombinant adenovirus infection in SH-SY5Y and IGR-N-91 cells. Untreated or uninfected cells (control of non-infected cells); cells infected by adenovirus expressing full-length p73{alpha} (Ad-TAp73{alpha}) or {Delta}Np73{alpha} (Ad-{Delta}Np73{alpha}) at MOI of 15 for 48 hours; cells infected by empty vector (Mock). Nearly 100% of treated cells showed p73 expression in the nucleus. (A) ICC of SH-SY5Y cells; (B) ICC of IGR-N-91 cells; (C) western blot of 1 µg of total protein extracts from SH-SY5Y cells (left panel), IGR-N-91 cells (middle panel), and of 50 µg endogenous total protein extracts (right panel); ß-actin was used as a protein loading control.





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