spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Cell cycle distribution showing differential patterns induced by TAp73{alpha} overexpression. (A) FACS. (B) Histogram representation of cell cycle population: Sub-G1 (black columns); G1 (white columns); S (hatched); G2/M (dots); data are means of three independent experiments ± s.d. (bars). The proportion of Ad-TAp73 SH-SY5Y neuroblasts (Ad-p73{alpha}) that underwent Sub-G1 phase increased considerably (60%) compared to Ad-{Delta}Np73{alpha} infected cells (10%) and to the non-infected cells and cells infected by empty vector (5% and 9%, respectively), indicating cells entering apoptosis. Ad-TAp73 IGR-N-91 cells exhibited a cell cycle distribution different from that of SH-SY5Y cells: more of the population underwent G1 phase (62% in treated versus 48% in control) leading to cell cycle arrest mediated by p21 activation as confirmed by western blot analysis shown in Fig. 3A.





Right arrow Return to article