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Fig. 6. Northern blots showing mRNA expression level of target genes in response to Ad-p73 SH-SY5Y and IGR-N-91 infected cells. (A) MDM2, BTG2, BAX and PUMA mRNA expression. It is noteworthy that in SH-SY5Y cells, BAX and PUMA mRNA expression is upregulated only in Ad-TAp73, indicating again the rapid apoptosis observed in such cells (see also FACS in Fig. 5). BTG2, the p53 target gene, known to be involved in the regulation of G1/S transition of the cell cycle (Duriez et al., 2002) is strongly stimulated in Ad-TAp73 but also stimulated in Ad-Np73 as compared to uninfected cells or cells infected by empty vector (Mock), or to Ad-TAp73 IGR-N-91 cells, Ad-Np73, whereas MDM2 mRNA level expression is highly stimulated in Ad-TAp73 IGR-N-91-infected cells compared to Ad-Np73- or control and empty vector-infected cells. Total RNA (10 µg) was prepared 48 hours after infection and submitted to northern blotting with appropriate cDNA probe; ethidium bromide staining of 28S and 18S rRNAs was used as an RNA loading control (A, lower panel). (B) Western blots showing protein expression of MDM2 and BAX. ß-Actin was used as protein loading control.

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