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Fig. 2. Serial section electron microscopic images, showing cross-sections of the basal body region of tua2-6; uni3-1 (A-F) and two different tua2-6; UNI3 cells (G-J and K-N). Scale bars 0.4 µm. (A) Distal region of a basal body with doublet microtubules. (B,C) Distal region of a basal body with doublet and triplet microtubule blades, triplet blades are indicated by asterisks. This basal body has three extended triplet blades. (D) Distal region of a basal body with both doublet and triplet microtubule blades, triplet blades are indicated by asterisks. Most of the triplet blades are only present at the distal end of the basal body. (E,F) Transition zone of a basal body as indicated by the stellate fiber. As in wild-type basal bodies, only doublet microtubule blades are observed in the transition zone. G) A mature basal body is present and a probasal body (lower right) with triplet microtubule blades; the angles of the blades are indicative of a probasal body rather than of a mature basal body. (H) A mature basal body and probasal body are separated by rootlet microtubules that form a cross-shaped pattern. (I) A mature basal body with the distal striated fibers that are present only at the distal end. Transition fibers emanate from the basal body at the distal end. (J) Transition fibers elongate in a more distal region. The majority of the blades still have triplet microtubules. (K) Triplet microtubules have become doublet microtubules. Transition fibers are still present and are elongated on the right of the basal body, but on the left they have become connectors to the membrane. (L) Doublet microtubules with connectors to the membrane. (M) Transition zone with the appearance of the central stellate fibers that form a central ring. (N) Outer doublet microtubules and central pair microtubules of the flagellar axoneme.
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